Faculty Sponsor
Lon Chubiz
Final Abstract for URS Program
Soil bacteria play essential roles in nutrient cycling, yet many abundant groups remain poorly characterized due to challenges in cultivation and genetic manipulation. One such organism is Edaphobacter flagellatus, a member of the Acidobacteriota frequently detected in soil environments but lacking well-developed experimental tools. To address this limitation, our laboratory has generated a genome-wide mutant library using the Tn5 transposon, enabling random disruption of genes across the population.
This project aims to establish culture conditions necessary to support the use of this library for functional genomic studies. In particular, defined minimal media can impose selective pressures that reveal gene function by requiring cells to synthesize essential metabolites. By combining controlled growth conditions with population-based sequencing approaches such as transposon sequencing, changes in mutant abundance can be used to infer gene fitness under different environmental conditions.
Presentation Type
Visual Presentation
Document Type
Article
Included in
Bacteriology Commons, Biology Commons, Environmental Microbiology and Microbial Ecology Commons, Genomics Commons, Molecular Genetics Commons