Document Type
Article
Abstract
The release of fatty acids from membrane lipids has been implicated in various plant processes, and the patatin-related phospholipases (pPLAs) constitute a major enzyme family that catalyzes fatty acid release. The Arabidopsis thaliana pPLA family has 10 members that are classified into three groups. Group 3 pPLAIII has four members but lacks the canonical lipase/esterase consensus catalytic sequences, and their enzymatic activity and cellular functions have not been delineated. Here, we show that pPLAIIIβ hydrolyzes phospholipids and galactolipids and additionally has acyl-CoA thioesterase activity. Alterations of pPLAIIIβ result in changes in lipid levels and composition. pPLAIIIβ-KO plants have longer leaves, petioles, hypocotyls, primary roots, and root hairs than wild-type plants, whereas pPLAIIIβ-OE plants exhibit the opposite phenotype. In addition, pPLAIIIβ-OE plants have significantly lower cellulose content and mechanical strength than wild-type plants. Root growth of pPLAIIIβ-KO plants is less sensitive to treatment with free fatty acids, the enzymatic products of pPLAIIIβ, than wild-type plants; root growth of pPLAIIIβ-OE plants is more sensitive. These data suggest that alteration of pPLAIIIβ expression and the resulting lipid changes alter cellulose content and cell elongation in Arabidopsis.
Publication Date
March 2011
Publication Title
The Plant Cell
Volume
23
Issue
3
First Page
1107
Last Page
1123
DOI
10.1105/tpc.110.081240
Recommended Citation
Wang, Xuemin; Li, Maoyin; Bahn, Sung Chul; Guo, Liang; Musgrave, William; Berg, Howard; and Welti, Ruth, "Patatin-Related Phospholipase pPLAIIIβ-Induced Changes in Lipid Metabolism Alter Cellulose Content and Cell Elongation in Arabidopsis" (2011). Biology Department Faculty Works. 172.
DOI: https://doi.org/10.1105/tpc.110.081240
Available at:
https://irl.umsl.edu/biology-faculty/172
Comments
© American Society of Plant Biologists.