Document Type
Article
Abstract
Diacyglycerol (DAG) is an important class of cellular lipid messengers, but its function in plants remains elusive. Here, we show that knockout of the Arabidopsis thaliana nonspecific phospholipase C (NPC4) results in a decrease in DAG levels and compromises plant response to abscisic acid (ABA) and hyperosmotic stresses. NPC4 hydrolyzes various phospholipids in a calcium-independent manner, producing DAG and a phosphorylated head group. NPC4 knockout (KO) plants display decreased ABA sensitivity in seed germination, root elongation, and stomatal movement and had decreased tolerance to high salinity and water deficiency. Overexpression of NPC4 renders plants more sensitive to ABA and more tolerant to hyperosmotic stress than wild-type plants. Addition of a short-chain DAG or a short-chain phosphatidic acid (PA) restores the ABA response of NPC4-KO to that of the wild type, but the addition of DAG together with a DAG kinase inhibitor does not result in a wild-type phenotype. These data suggest that NPC4-produced DAG is converted to PA and that NPC4 and its derived lipids positively modulate ABA response and promote plant tolerance to drought and salt stresses.
Publication Date
July 2010
Publication Title
The Plant Cell
Volume
22
First Page
2642
Last Page
2659
DOI
10.1105/tpc.109.071720
Recommended Citation
Wang, Xuemin; Peters, Carlotta; Li, Maoyin; Narasimhan, Rama; Roth, Mary; and Welti, Ruth, "Nonspecific Phospholipase C NPC4 Promotes Responses to Abscisic Acid and Tolerance to Hyperosmotic Stress in Arabidopsis" (2010). Biology Department Faculty Works. 183.
DOI: https://doi.org/10.1105/tpc.109.071720
Available at:
https://irl.umsl.edu/biology-faculty/183
Comments
© American Society of Plant Biologists.