Document Type
Article
Abstract
We present a single-cell motility assay, which allows the quantification of bacterial swimming in a well-controlled environment, for durations of up to an hour and with a temporal resolution greater than the flagellar rotation rates of ∼100 Hz. The assay is based on an instrument combining optical tweezers, light and fluorescence microscopy, and a microfluidic chamber. Using this device we characterized the long-term statistics of the run-tumble time series in individual Escherichia coli cells. We also quantified higher-order features of bacterial swimming, such as changes in velocity and reversals of swimming direction.
Publication Date
January 2009
Publication Title
Nature Methods
ISSN
15487091
Volume
6
Issue
11
First Page
831
Last Page
835
DOI
https://doi.org/10.1038/nmeth.1380
Recommended Citation
Chubiz, Lon; Min, Taejin; Mears, Patrick; Rao, Christopher; Golding, Ido; and Chemla, Yann, "High-Resolution, Long-Term Characterization of Bacterial Motility Using Optical Tweezers" (2009). Biology Department Faculty Works. 100.
DOI: https://doi.org/https://doi.org/10.1038/nmeth.1380
Available at:
https://irl.umsl.edu/biology-faculty/100