The RNA-Binding Site of Bacteriophage Qβ Coat Protein

Authors

Francis Lim, University of New Mexico
Marc Spingola, University of New Mexico
David Peabody, University of New Mexico

Document Type

Article

Abstract

The coat proteins of the RNA bacteriophages Qβ and MS2 are specific RNA binding proteins. Although they possess common tertiary structures, they bind different RNA stem loops and thus provide useful models of specific protein-RNA recognition. Although the RNA-binding site of MS2 coat protein has been extensively characterized previously, little is known about Qβ. Here we describe the isolation of mutants that define the RNA-binding site of Qβ coat protein, showing that, as with MS2, it resides on the surface of a large β-sheet. Mutations are also described that convert Qβ coat protein to the RNA binding specificity of MS2. The results of these and other studies indicate that, although they bind different RNAs, the binding sites of the two coat proteins are sufficiently similar that each is easily converted by mutation to the RNA binding specificity of the other.

Publication Date

December 1996

Publication Title

Journal of Biological Chemistry

Volume

271

Issue

50

First Page

31839

Last Page

31845

DOI

10.1074/jbc.271.50.31839

Recommended Citation

Lim, Francis; Spingola, Marc; and Peabody, David, "The RNA-Binding Site of Bacteriophage Qβ Coat Protein" (1996). Biology Department Faculty Works. 158.
DOI: https://doi.org/10.1074/jbc.271.50.31839
Available at: https://irl.umsl.edu/biology-faculty/158