Document Type

Article

Keywords

camelina, patatin‐related phospholipase III δ, seed oil, cellulose, plant growth

Abstract

Camelina sativa is a Brassicaceae oilseed species being explored as a biofuel and industrial oil crop. A growing number of studies have indicated that the turnover of phosphatidylcholine plays an important role in the synthesis and modification of triacylglycerols. This study manipulated the expression of a patatin‐related phospholipase AIIIδ (pPLAIII δ) in camelina to determine its effect on seed oil content and plant growth. Constitutive overexpression of pPLAIII δ under the control of the constitutive cauliflower mosaic 35S promoter resulted in a significant increase in seed oil content and a decrease in cellulose content. In addition, the content of major membrane phospholipids, phosphatidylcholine and phosphatidylethanolamine, in 35S::pPLAIII δ plants was increased. However, these changes in 35S::pPLAIII δ camelina were associated with shorter cell length, leaves, stems, and seed pods and a decrease in overall seed production. When pPLAIII δ was expressed under the control of the seed specific, β‐conglycinin promoter, the seed oil content was increased without compromising plant growth. The results suggest that pPLAIII δ alters the carbon partitioning by decreasing cellulose content and increasing oil content in camelina.

Publication Date

January 2014

Publication Title

Plant Biotechnology Journal

Volume

13

Issue

6

First Page

766

Last Page

778

DOI

10.1111/pbi.12304

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