2-acylamido analogues of n-acetylglucosamine prime formation of chitin oligosaccharides by yeast chitin synthase 2

Authors

Jacob Gyore, School of Molecular and Cellular Biology
Archana R. Parameswar, University of Missouri-St. Louis
Carleigh F.F. Hebbard, School of Molecular and Cellular Biology
Younghoon Oh, University of Pennsylvania Perelman School of Medicine
Erfei Bi, University of Pennsylvania Perelman School of Medicine
Alexei V. Demchenko, University of Missouri-St. LouisFollow
Neil P. Price, USDA ARS National Center for Agricultural Utilization Research
Peter Orlean, School of Molecular and Cellular Biology

Document Type

Article

Abstract

Background: Chitin synthases are stimulated by N-acetylglucosamine (GlcNAc). Results: GlcNAc and 2-acylamido analogues of GlcNAc stimulate formation of chitin oligosaccharides by yeast chitin synthase, and GlcNAc is transferred to the 2-acylamido analogues. Conclusion: Chitin synthases use GlcNAc analogues as primers and transfer one GlcNAc at a time. Significance: Results are new insights into polysaccharide synthase mechanism and suggest ways of synthesizing novel modified polysaccharides. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

Publication Date

1-1-2014

Publication Title

Journal of Biological Chemistry

ISSN

00219258

E-ISSN

1083351X

Volume

289

Issue

18

First Page

12835

Last Page

12841

DOI

10.1074/jbc.M114.550749

PubMed ID

24619411

Funding Number

GM077170

Funding Sponsor

National Institute of General Medical Sciences

Recommended Citation

Gyore, Jacob; Parameswar, Archana R.; Hebbard, Carleigh F.F.; Oh, Younghoon; Bi, Erfei; Demchenko, Alexei V.; Price, Neil P.; and Orlean, Peter, "2-acylamido analogues of n-acetylglucosamine prime formation of chitin oligosaccharides by yeast chitin synthase 2" (2014). Chemistry & Biochemistry Faculty Works. 109.
DOI: https://doi.org/10.1074/jbc.M114.550749
Available at: https://irl.umsl.edu/chemistry-faculty/109