Document Type

Article

Abstract

The brains of Alzheimer’s disease (AD) patients contain large numbers of amyloid plaques that are rich in fibrils composed of 40- and 42-residue amyloid-β (Aβ) peptides. Several lines of evidence indicate that fibrillar Aβ and especially soluble Aβ aggregates are important in the etiology of AD. Recent reports also stress that amyloid aggregates are polymorphic and that a single polypeptide can fold into multiple amyloid conformations. Here we demonstrate that Aβ-(1–40) can form soluble aggregates with predominant β-structures that differ in stability and morphology. One class of aggregates involved soluble Aβ protofibrils, prepared by vigorous overnight agitation of monomeric Aβ-(1–40) at low ionic strength. Dilution of these aggregation reactions induced disaggregation to monomers as measured by size exclusion chromatography. Protofibril concentrations monitored by thioflavin T fluorescence decreased in at least two kinetic phases, with initial disaggregation (rate constant k1) followed by a much slower secondary phase. Incubation of the reactions without agitation resulted in less disaggregation at slower rates, indicating that the protofibrils became progressively more stable over time. In fact, protofibrils isolated by size exclusion chromatography were completely stable and gave no disaggregation. A second class of soluble Aβ aggregates was generated rapidly (<10 min) in buffered 2% hexafluoroisopropanol (HFIP). These aggregates showed increased thioflavin T fluorescence and were rich in β-structure by circular dichroism. Electron microscopy and atomic force microscopy revealed initial globular clusters that progressed over several days to soluble fibrous aggregates. When diluted out of HFIP, these aggregates initially were very unstable and disaggregated completely within 2 min. However, their stability increased as they progressed to fibers. Relative to Aβ protofibrils, the HFIP-induced aggregates seeded elongation by Aβ monomer deposition very poorly. The techniques used to distinguish these two classes of soluble Aβ aggregates may be useful in characterizing Aβ aggregates formed in vivo.

Publication Date

January 2005

Publication Title

The Journal of Biological Chemistry

Volume

280

Issue

4

First Page

2471

Last Page

2480

DOI

10.1074/jbc.M410553200

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