Document Type
Article
Abstract
Established transfection methodology often uses commercial reagents, which must be formed into liposomes in a sequence of about half a dozen steps. The simplified method reported here is a direct lipid mixing approach that requires fewer steps, less manipulation, and is less time-consuming. Results are comparable to those obtained with more commonly used methods, as judged by a variety of analytical techniques and by comparisons of transfection results. The method reported here may be applied to non-liposome-forming compounds, thereby greatly expanding the range of structures that can be tested for transfection ability.
Publication Date
September 2016
Publication Title
Scientific Reports
Volume
6
Issue
1
First Page
1
Last Page
12
DOI
10.1038/srep27662
Recommended Citation
Meisel, Joseph and Gokel, George, "A Simplified Direct Lipid Mixing Lipoplex Preparation: Comparison of Liposomal-, Dimethylsulfoxide-, and Ethanol-Based Methods" (2016). Chemistry & Biochemistry Faculty Works. 47.
DOI: https://doi.org/10.1038/srep27662
Available at:
https://irl.umsl.edu/chemistry-faculty/47