Document Type
Article
Abstract
A 45-kDa polypeptide preferentially present in neuronal membranes was previously identified as a subunit of a binding (or receptor) protein for several phospholipase A2 variants with neurotoxicity, including crotoxin, by chemical cross-linking experiments (Yen, C.-H., and Tzeng, M.-C.(1991) Biochemistry 30, 11473-11477). The binding of crotoxin to this receptor protein was completely suppressed by sufficient F22Y, a mutated bovine pancreatic phospholipase A2 generated by site-directed mutagenesis of Phe of the wild-type enzyme to Tyr. The IC of this inhibition was estimated to be 1 μM. In sharp contrast, the wild-type enzyme gave no effect even at 50 μM. This mutation resulted in only minor and localized structural perturbations with little effect on enzymatic activity. Other phospholipase A2 molecules capable of competing with crotoxin for this binding invariably have Tyr at this position. It was concluded that this Tyr residue is an important determinant for the binding of a number of phospholipase A2 variants to the 45-kDa receptor.
Publication Date
2-3-1995
Publication Title
Journal of Biological Chemistry
Volume
270
Issue
5
First Page
2120
Last Page
2123
DOI
10.1074/jbc.270.5.2120
Recommended Citation
Tzeng, Mu-Chin; Yen, Chon-Ho; Hseu, Ming-Jhy; Dupureur, Cynthia M.; and Tsai, Ming-Daw, "Conversion of Bovine Pancreatic Phospholipase A at a Single Site into a Competitor of Neurotoxic Phospholipases A by Site-directed Mutagenesis" (1995). Educator Preparation & Leadership Faculty Works. 116.
DOI: https://doi.org/10.1074/jbc.270.5.2120
Available at:
https://irl.umsl.edu/epir/116