Document Type
Article
Abstract
Dramatic improvements in ribozyme mimics have been achieved by employing the principles of small molecule catalysis to the design of macromolecular, biomimetic reagents. Ribozyme mimics derived from the ligand 2,9-dimethylphenanthroline (neocuproine) show at least 30-fold improvements in efficiency at sequence-specific RNA cleavage when compared with analogous o-phenanthroline- and terpyridine-derived reagents. The suppression of hydroxide-bridged dimers and the greater activation of coordinated water by Cu(II) neocuproine (compared with the o-phananthroline and terpyridine complexes) better allow Cu(II) to reach its catalytic potential as a biomimetic RNA cleavage agent. This work demonstrates the direct mapping of molecular design principles from small-molecule cleavage to macromolecular cleavage events, generating enhanced biomimetic, sequence-specific RNA cleavage agents.
Publication Date
5-15-2001
Publication Title
Nucleic Acids Research
Volume
29
Issue
10
First Page
2199
Last Page
2204
DOI
10.1093/nar/29.10.2199
Recommended Citation
Putnam, William C.; Daniher, Andrew T.; Trawick, Bobby N.; and Bashkin, James K., "Efficient new ribozyme mimics: direct mapping of molecular design principles from small molecules to macromolecular, biomimetic catalysts" (2001). Educator Preparation & Leadership Faculty Works. 73.
DOI: https://doi.org/10.1093/nar/29.10.2199
Available at:
https://irl.umsl.edu/epir/73
