Document Type
Thesis
Degree
Master of Science
Major
Biochemistry & Biotechnology
Date of Defense
11-20-2009
Graduate Advisor
Wendy M. Olivas, PhD
Co-Advisor
Dr Colin MacDiarmid
Committee
Dr Wendy Olivas
Dr Lisa Schechter
Abstract
Magnesium is required for many essential processes and pathways, including protein synthesis and energy metabolism. In the yeast Saccharomyces cerevisiae, Mg2+ uptake across the plasma membrane primarily occurs via the magnesium transporter Alr1. Preliminary data had shown that Alr1-HA accumulation was decreased by Mg. Here I show that the epitope tag was responsible for this differential accumulation of Alr1, as native or N-terminally tagged Alr1 displayed much less differential accumulation. Immunoblotting and Ni2+ uptake assays indicated that both Mg-deficiency and the mnr2 mutation increased Alr1 activity, but under conditions where native protein accumulation was little affected. Since Mg-dependent regulation of Alr system activity and Alr1 protein accumulation did not correlate, I investigated the importance of the post-translational modification of Alr1 to regulation. These studies suggested that the post-translational modification of Alr1 and the C-terminal domain of the protein may be important for regulation.
OCLC Number
833393078
Recommended Citation
Lim, Phaik Har, "Regulation of the Alr1 transporter by Mg supply" (2009). Theses. 4.
https://irl.umsl.edu/thesis/4